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Selenotest ELISA kit說明書

更新時間：2016-10-25 點擊次數(shù)：2901

immunochemical inligence GmbH?是現(xiàn)在*生產selenoprotein P?試劑盒的廠家，該試劑盒是使用ELISA方法來測定人血漿中selenoprotein P蛋白含量，方便檢測

selenoprotein P (SEPP1)研究背景

亞細胞定位: Secreted.

組織特異性: Made in the liver and heart and secreted into the plasma. It is also found in the kidney.

翻譯后修飾: Phosphorylation sites are present in the extracellular medium.

相似的序列: Belongs to the selenoprotein P family.

品牌	immunochemical inligence GmbH
產品貨號	STE
產品名稱	Selenotest ELISA kit
用途	檢測人類血漿selenoprotein P (SEPP1)蛋白含量
包裝	96孔板
檢測方法	比色法 Colorimetric (450 nm)
樣品需要量	5 – 10 µL
采樣類型	血漿
檢測時間	5 – 6小時
靈敏度	‹ 8 µg/L (about 500-fold below the serum level of healthy individuals)
檢測范圍	Approx. 5 – 800 µg/L
定量范圍	Approx. 10 – 400 µg/L (corresponds to 0.2 – 8 mg/L in undiluted sample)
試劑盒保存溫度	Refrigerated (2 – 8度)
保質期	6個月到中國客戶手上至少3個月
試劑盒尺寸(L×W×H)	200 × 150 × 95毫米
試劑盒重量	400g
生產地址	德國
價格	750歐元，7500RMB
到中國運輸費用	2000元
到中國的時間	4周左右
代理商	上海起發(fā)實驗試劑有限公司
關鍵詞	SELENOP selenoprotein P SEPP1
成分組成：
包被好的96孔板	1：Pre-coated, pre-blocked 96 well microplate (1 plate, ready-for-use)
凍干血漿標準品 8管	2：Serum Calibrators, lyophilized (8 tubes, 0.5 mL each)
陽性對照 3管	3：Serum Controls, lyophilized (3 tubes, 0.5 mL each)
陰性對照 1管	4：Assay Blank, lyophilized (1 tube, 0.5 mL)
重建緩沖液 8ML 一瓶	5：Reconstitution Buffer (1 bottle, 8 mL)
樣品稀釋液20ml一瓶	6：Sample Dilution Buffer (1 bottle, 20 mL)
生物素標記抗體125ul	7:Concentrated biotin-conjugated Detection Antibody (DAB), liquid (1 tube, 0.125 mL)
鏈霉親和素過氧化物酶復合物	8:Concentrated Streptavidin-Peroxidase Complex (POD), liquid (1 tube, 0.125 mL)
DAB稀釋液11ML	9:DAB Dilution Buffer (1 bottle, 11 mL)
POD稀釋液11ml	10:POD Dilution Buffer (1 bottle, 11 mL)
20x洗滌緩沖液25ml	11:Washing Buffer, 20× concentrate (1 bottle, 25 mL)
POD底物溶液11ml	12:POD Substrate Solution (1 bottle, 11 mL)
終止液11ml	13:Stop Solution (1 bottle, 11 mL)
封板膜4張	14:Plate Cover Foil (4 pieces)
操作指南一份英文	15:Instructions For Use
產品清單一份	16:Product Specification Sheet
質量檢查報告一份	17:Quality Control Certificate

需要額外準備的產品
移液器及配套吸頭	Calibrated, adjustable precision single- and multichannel pipettes with disposable plastic tips
各種樣品管或者微孔板	Polypropylene 96 well microplate or tubes for diluting samples
混勻器	Vortex mixer
離心機	Centrifuge for microtubes
洗板機	Automated microplate washer (option)
搖床	Microplate shaker
分光光度計	Microplate reader capable measuring absorbance at 450 nm
吸水紙	Absorbent paper towels
純凈水	Laboratory water (type 1 or 2)
相關軟件	Data analysis and graphing software

References（引用文獻）

[1] Hybsier S, Wu Z, Schulz T, Strasburger CJ, Köhrle J, Minich WB, Schomburg L. Establishment and characterization of a new ELISA for selenoprotein P. Perspectives in Science. 2015 Feb;3(1-4):23-4. http://dx.doi.org/10.1016/j.pisc.2014.11.013

[2] Ballihaut G, Kilpatrick LE, Kilpatrick EL, Davis WC. Multiple forms of selenoprotein P in a candidate human plasma standard reference material. Metallomics. 2012 Jun;4(6):533-8. http://dx.doi.org/10.1039/c2mt20059g

[3] Ballihaut G, Kilpatrick LE, Davis WC. Detection, identification, and quantification of selenoproteins in a candidate human plasma standard reference material. Anal Chem. 2011 Nov;83(22):8667-74. http://dx.doi.org/10.1021/ac2021147

資料下載：

Poster Presentations

The Selenotest ELISA was first presented at the GMS Jahrestagung 2013 (Gesellschaft für Mineralstoffe und Spurenelemente) and at the 10th International Symposium on Selenium in Biology and Medicine 2013 in Berlin, Germany:

[pdf, 199KB] Hybsier S, Wu Z, Schulz T, Strasburger CJ, Köhrle J, Minich WB, Schomburg L. Establishment and characterization of a new ELISA for selenoprotein P. Poster Presentation at 10^thInternational Symposium on Selenium in Biology and Medicine 2013, Berlin.

Instructions For Use

[pdf] Arbeitsanleitung (Version 3, DE) [pdf] Instructions for use (Version 3, EN)

Product Specification Sheets

[pdf] Lot:MSP013001 [pdf] Lot:MSP013002 [pdf] Lot:MSP014001 [pdf] Lot:MSP015001

Quality Control (QC) Certificates