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LigaTrap®Technologies開(kāi)發(fā)了一種新穎的專有技術(shù)親和力配體組合，專門(mén)用于從各種物種中純化單克隆和多克隆免疫球蛋白。除了物種特異性配體外，優(yōu)點(diǎn)包括能夠在比蛋白質(zhì)a和G（pH≤3.0）更溫和的pH（pH 4.0）下洗脫結(jié)合抗體，從而降低pH敏感抗體沉淀和失活的可能性。我們的目標(biāo)是利用優(yōu)于目前市場(chǎng)上可用的配體來(lái)產(chǎn)生高純度抗體。LigaTrap®團(tuán)隊(duì)對(duì)目前市場(chǎng)上的凈化產(chǎn)品進(jìn)行了廣泛的并行測(cè)試。LigaTrap®團(tuán)隊(duì)還擴(kuò)展了我們的能力，包括我們的專家流程開(kāi)發(fā)和下游優(yōu)化服務(wù)。
LigaTrap®正積極與治療行業(yè)快速發(fā)展的技術(shù)和平臺(tái)合作。
IgM Purification Resin and Columns
| Part # | Name | Amount | Price |
| LT-143KIT | LigaTrap Human IgM Kit | 1 Kit | $379.00 |
| LT-143 | LigaTrap Human IgM Resin | 5 ml | $699.00 |
| LT-143-1x1ml | LigaTrap® Human IgM Purification Column | 1 ml | $149.00 |
| LT-143-1x5ml | LigaTrap® Human IgM Purification Column | 1 x 5 ml | $699.00 |
| LT-143-3x1ml | LigaTrap® Human IgM Purification Column | 3 x 1 ml | $429.00 |
IgM的研究始于1937年的報(bào)告，即用肺炎球菌多糖超免疫的馬產(chǎn)生的抗體比典型的兔γ-球蛋白大得多，分子量為990,000道爾頓。根據(jù)其大尺寸，新抗體初被稱為γ-巨球蛋白，然后在隨后的術(shù)語(yǔ)中稱為“宏”的IgM-M。
IgM是大的抗體，并且是對(duì)抗原初次暴露時(shí)出現(xiàn)的一個(gè)抗體。就已研究的人類和其他哺乳動(dòng)物而言，脾臟是產(chǎn)生特定IgM的主要部位，脾臟是負(fù)責(zé)產(chǎn)生抗體的漿母細(xì)胞所在。
IgM單克隆抗體越來(lái)越適合用作治療工具，同時(shí)在其研究和診斷應(yīng)用中仍然很受歡迎。蛋白A和G色譜法繼續(xù)著生命科學(xué)行業(yè)提純IgG，但是蛋白A / G證明不適合IgM純化。
除了提高產(chǎn)量和純度，LigaTrap®人IgM純化樹(shù)脂（LT-143）還解決了許多與標(biāo)準(zhǔn)親和配體有關(guān)的不足，包括與其他常見(jiàn)純化方法相關(guān)的蛋白水解和重復(fù)消毒的挑戰(zhàn)。
免責(zé)聲明：由于可能與其他免疫球蛋白發(fā)生交叉反應(yīng)，因此不建議在血清的純化應(yīng)用中使用LigaTrap Human IgA純化樹(shù)脂。
使用IgM純化樹(shù)脂的主要競(jìng)爭(zhēng)者從含有5％FBS的CHO培養(yǎng)基中純化人IgM。LigaTrap®LT-143樹(shù)脂使用標(biāo)準(zhǔn)色譜條件。IgM純化樹(shù)脂的主要競(jìng)爭(zhēng)者使用了三種不同的制造商推薦的結(jié)合條件。
可比性
SDS頁(yè)面
LigaTrap純化試劑盒和樹(shù)脂產(chǎn)品目錄
LigaTrap純化試劑盒和樹(shù)脂 方便易用。只需將樣品添加到樹(shù)脂中，混合并離心。LigaTrap®純化方案不僅易于遵循，而且可以快速簡(jiǎn)便地回收純化的抗體，以便在終用途分析中進(jìn)行篩選。可以同時(shí)處理多個(gè)樣品，從而 快速 篩選抗體的產(chǎn)生。LigaTrap®旋轉(zhuǎn)柱僅需使用移液器和離心機(jī)，無(wú)需設(shè)備。每個(gè)旋轉(zhuǎn)柱多可以進(jìn)行10次純化，每個(gè)試劑盒都可以進(jìn)行100多次純化，從而使LigaTrap®成為一種經(jīng)濟(jì)高效的抗體純化方法。
LigaTrap®Technologies是*、新穎且獲得的肽/肽配體技術(shù)的創(chuàng)新者。我們強(qiáng)大的親和力配體組合具有更長(zhǎng)的生命周期，需要溫和的pH洗脫來(lái)產(chǎn)生高純度抗體。
| Part # | Name | Amount | Price |
| LT-136KIT | LigaTrap Goat IgG Kit | 1 Kit | $294.00 |
| LT-136 | LigaTrap Goat IgG Resin | 5 ml | $450.00 |
| LT-136-1x1ml | LigaTrap® Goat IgG Purification Column | 1 ml | $100.00 |
| LT-136-1x5ml | LigaTrap® Goat IgG Purification Column | 1 x 5 ml | $475.00 |
| LT-136-3x1ml | LigaTrap® Goat IgG Purification Column | 3 x 1 ml | $295.00 |
| LT-095KIT | LigaTrap Human IgG Kit | 1 Kit | $294.00 |
| LT-095 | LigaTrap Human IgG Resin | 5 ml | $450.00 |
| LT-095-1x1ml | LigaTrap® Human IgG Purification Column | 1 ml | $100.00 |
| LT-095-1x5ml | LigaTrap® Human IgG Purification Column | 1 x 5 ml | $475.00 |
| LT-095-3x1ml | LigaTrap® Human IgG Purification Column | 3 x 1 ml | $295.00 |
| LT-137KIT | LigaTrap Mouse IgG Kit | 1 Kit | $294.00 |
| LT-137 | LigaTrap Mouse IgG Resin | 5 ml | $450.00 |
| LT-137-1x1ml | LigaTrap® Mouse IgG Purification Column | 1 ml | $100.00 |
| LT-137-1x5ml | LigaTrap® Mouse IgG Purification Column | 1 x 5 ml | $475.00 |
| LT-137-3x1ml | LigaTrap® Mouse IgG Purification Column | 3 x 1 ml | $295.00 |
| LT-139KIT | LigaTrap Rabbit IgG Kit | 1 Kit | $294.00 |
| LT-139 | LigaTrap Rabbit IgG Resin | 5 ml | $450.00 |
| LT-139-1x1ml | LigaTrap® Rabbit IgG Purification Column | 1 ml | $100.00 |
| LT-139-1x5ml | LigaTrap® Rabbit IgG Purification Column | 1 x 5 ml | $475.00 |
| LT-139-3x1ml | LigaTrap® Rabbit IgG Purification Column | 3 x 1 ml | $295.00 |
| LT-138KIT | LigaTrap Rat IgG Kit | 1 Kit | $294.00 |
| LT-138 | LigaTrap Rat IgG Resin | 5 ml | $450.00 |
| LT-138-1x1ml | LigaTrap® Rat IgG Purification Column | 1 ml | $100.00 |
| LT-138-1x5ml | LigaTrap® Rat IgG Purification Column | 1 x 5 ml | $475.00 |
| LT-138-3x1ml | LigaTrap® Rat IgG Purification Column | 3 x 1 ml | $295.00 |
| LT-141KIT | LigaTrap Sheep IgG Kit | 1 Kit | $294.00 |
| LT-141 | LigaTrap Sheep IgG Resin | 5 ml | $450.00 |
| LT-141-1x1ml | LigaTrap® Sheep IgG Purification Column | 1 ml | $100.00 |
| LT-141-1x5ml | LigaTrap® Sheep IgG Purification Column | 1 x 5 ml | $475.00 |
| LT-141-3x1ml | LigaTrap® Sheep IgG Purification Column | 3 x 1 ml | $295.00 |
| LT-142KIT | LigaTrap Chicken IgY Kit | 1 Kit | $349.00 |
| LT-142 | LigaTrap Chicken IgY Resin | 5 ml | $555.00 |
| LT-142-1x1ml | LigaTrap® Chicken IgY Purification Column | 1 ml | $125.00 |
| LT-142-1x5ml | LigaTrap® Chicken IgY Purification Column | 1 x 5 ml | $595.00 |
| LT-142-3x1ml | LigaTrap® Chicken IgY Purification Column | 3 x 1 ml | $365.00 |
| LT-143KIT | LigaTrap Human IgM Kit | 1 Kit | $379.00 |
| LT-143 | LigaTrap Human IgM Resin | 5 ml | $699.00 |
| LT-143-1x1ml | LigaTrap® Human IgM Purification Column | 1 ml | $149.00 |
| LT-143-1x5ml | LigaTrap® Human IgM Purification Column | 1 x 5 ml | $699.00 |
| LT-143-3x1ml | LigaTrap® Human IgM Purification Column | 3 x 1 ml | $429.00 |
| LT-144KIT | LigaTrap Llama IgG Kit | 1 Kit | $294.00 |
| LT-144 | LigaTrap Llama IgG Resin | 5 ml | $450.00 |
| LT-144-1x1ml | LigaTrap® Llama IgG Purification Column | 1 ml | $100.00 |
| LT-144-1x5ml | LigaTrap® Llama IgG Purification Column | 1 x 5 ml | $475.00 |
| LT-144-3x1ml | LigaTrap® Llama IgG Purification Columns | 3 x 1 ml | $295.00 |
| LT-145KIT | LigaTrap Mouse IgM Kit | 1 Kit | $379.00 |
| LT-145 | LigaTrap Mouse IgM Resin | 5 ml | $669.00 |
| LT-145-1x1ml | LigaTrap® Mouse IgM Purification Column | 1 ml | $149.00 |
| LT-145-1x5ml | LigaTrap® Mouse IgM Purification Column | 1 x 5 ml | $699.00 |
| LT-145-3x1ml | LigaTrap® Mouse IgM Purification Column | 3 x 1 ml | $429.00 |
| LT-146KIT | LigaTrap Human IgA Kit | 1 Kit | $294.00 |
| LT-146 | LigaTrap Human IgA Resin | 5 ml | $450.00 |
| LT-146-1x1ml | LigaTrap® Human IgA Purification Column | 1 ml | $100.00 |
| LT-146-1x5ml | LigaTrap® Human IgA Purification Column | 1 x 5 ml | $475.00 |
| LT-146-3x1ml | LigaTrap® Human IgA Purification Column | 3 x 1 ml | $295.00 |
| LT-147Kit | LigaTrap Rat IgM Kit | 1 Kit | $379.00 |
| LT-147 | LigaTrap Rat IgM Resin 5ml | 5 ml | $669.00 |
| LT-147-1x1ml | LigaTrap® Rat IgM Purification Column | 1 ml | $149.00 |
| LT-147-1x5ml | LigaTrap® Rat IgM Purification Column | 1 x 5 ml | $699.00 |
| LT-147-3x1ml | LigaTrap® Rat IgM Purification Column | 3 x 1 ml | $429.00 |
| BU-123-FP | LigaTrap Elution Buffer | 125ml | $22.00 |
| BU-124-FP | LigaTrap Regeneration Buffer | 50ml | $22.00 |
| BU-125-FP | LigaTrap Neutralization Buffer | 15ml | $22.00 |
| BU-126-FP | LigaTrap Storage Buffer | 50 ml | $22.00 |
| BU-131-FP | LigaTrap Sample Diluent 2.0 | 15 ml | $22.00 |
| BU-132-FP | LigaTrap Equilibration/Wash Buffer 2.0 | 250ml | $27.00 |
| BU-123-500-FP | LigaTrap Elution Buffer | 500ml | $60.00 |
| BU-124-500-FP | LigaTrap Regeneration Buffer | 500 ml | $125.00 |
| BU-125-250-FP | LigaTrap Neutralization Buffer | 250 ml | $149.00 |
| BU-126-500-FP | LigaTrap Storage Buffer | 500 ml. | $125.00 |
| BU-131-250-FP | LigaTrap Sample Diluent 2.0 | 250 ml. | $235.00 |
| BU-132-1-FP | LigaTrap Equilibration/Wash Buffer 2.0 | 1L | $85.00 |
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